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Molecular Tests

Terms

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What does FLT-3 stand for?
fmr-like tyrosine kinase 3
How many exons does FLT-3 have?
24
How many base pairs are in the FLT-3 gene?
96 kbp
What types of mutations does the FLT-3 assay test for?
and Internal Tandem Duplication in exon 11 (the juxtamembrane domain); and the D835 mutation (activation loop of the kinase domain)
What type of cancer is the FLT-3 mutation associated with?
Acute myeloid leukemia?
What is the percent of AML cases that are FLT-3 positive?
20-30%
What is the effect of being FLT-3 positive on a AML patient's prognosis/treatment?
Poor prognosis; more aggressive treatment necesssary; more likely to relapse;
What is Hemochromatosis
a hereditary disease characterized by improper processing by the body of dietary iron which causes iron to accumulate in a number of body tissues, eventually causing organ dysfunction. It is the main iron
What are the different possible HFE mutations leading to Hemochromatosis?     

C282Y

H63D

S65C
 

How many types of HPV are considered high risk for cervical cancer?

13 total,

4 high risk

What are the 13 types

 

16 / 18 / 31 / 33 / 35 / 39 / 45 / 51 / 52 / 56 / 58 / 

59 / 68 

What is the progression from HPV infection to cervical cancer?            
HPV infection -> cervical dyskaryosis,-> cercical cancer
Where is the HFE gene located ?
On the short arm of chromosome 6     
What is the JAK-2 mutation?

Janus kinase 2 (Jak2) is essential for signal transduction by mediating tyrosine phosphorylation. 

Jak2 mutation by way of chromosomal translocations in hematopoietic tumors and mutations within the pseudokinase domain in a spectrum of myelo

What is a chromosomal translocation? (review)

a chromosomal abnormality caused by rearrangment of parts between non-homologous chromosomes.

 

Clinically, it is detected through cytogenetic methods (ie a karytype of affected cells.

What are the two main types of chromosomal translocations?     

Non-robertsonian

 

Robertsonian 

 

translocations can also bebalanced or unbalanced 

Non-robertsonian
reciprocal between two chromsomes, causes normal phenotypes, may cause nondisjunction in future offspring
Robertsonian
involves two acrocentric chromosomes that have fused near the center, causing a karyotype of 45 chromsomes instead of 46

 

Deck Info

18

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