general facts
Terms
undefined, object
copy deck
- diameter of DNA helix looking down from top view
- 20 angstroms
- nucleoside
- base plus a sugar
- RNA nucleoside units
-
Adenosine
Guanosine
Cytidine
Uridine - DNA nucleosides
-
deoxyadenosine
deoxyguanosine
deoxycytidine
thymidine - configuration of glycosidic linkage
- Beta - above plane of sugar
- what is a nucleotide
- nucleoside joined to one or more phosphate groups by ester linkage
- 4 nucleotide units in DNA
-
deoxyadenylate
deoxyguanylate
deoxycytidylate
thymidylate - how much space seperates adjacent bases in DNA helix
- 3.4 angstroms
- how many angstroms per repeate/turn of helix
- 34 angstroms
- how many bases per turn of DNA helix
- 10
- definition of a gene
- a region of DNA (sometimes RNA) that encodes a specific biological function
- genome
- total complement of genes in an organism virus or organell
- chromosome
-
multiple-DNA molecules
(have more than one gene on them) - how many bp in e.coli genome
-
5 x 10^6
5,000,000 - how many DNA bp in yeast genome
-
10 x 10^7
100,000,000 - how many DNA bp in human genome
-
3 x 10^9
3 billion - anomer of sugar in DNA or RNA is always
- beta-furanose
- how are nucleotides usually named
- nucleoside phosphates
- oside's for ADENINE
-
RNA: adenosine
DNA: deoxyadenosine - nucleoside nomenclature for guanidine
-
RNA: guanosine
DNA: deoxyguanosine - nucleoside nomenclature for cytosine
-
RNA: cytidine
DNA: deoxycytidine - ribonucleotide nomenclature for adenine
-
RNA: adenylate AMP
DNA: deoxyadenylate dAMP - What type of bonds are between bases in the DNA helix
- hydrogen bonds
- what type of symmetry is between base pairs
- pseudosymmetry
- 3 different conformations of the DNA Helix:
- ABZ
- why are there A B and Z conformations
- different puckerings of ribose units
-
how many bp per turn of
-A DNA
-B Dna
-Z DNA -
11 -- A is shorter and fatter
10 -- this is normal DNA
12 -- this is longer an thinner - what are DNA major/minor grooves important for
- sequence specific recognition
- Supercoiling
- writhing/coiling of the DNA helix
- what helps strands seperate in DNA replication?
- negative supercoiling -- slight underwinding of DNA.
-
what takes up more space:
-Supercoiled
-Relaxed DNA? - Relaxed
- essence of semiconservative replication
- parent molecule (2 complementary chains) gives 2 daughter molecules, each containing half of the parent
- What are the 2 models for DNA replication:
-
Conservative: parent chain not present in daughter molecule - its brand new
semiconservative - parent donates half of its chain to two duaghters