Procedure
Terms
undefined, object
copy deck
- Order of microscope powers
-
A. Low power
B. High-dry
C. Oil immersion - What are the two things you do at low power in a diff?
-
1. Determine nonrandom cell distribution - a. fringe analysis
b. rouleaux/agglutination
c. platelet clumping
d. look for artifact
2. Select the best area for a diff - What occurs at the High-dry power in a diff?
- WBC estimate.
- How do you do a WBC estimate?
-
1. Area: RBC overlapping slightly
2. Average #WBCs in 5 fields.
3. Multiply x 2000 = WBC estimate
4. See if estimate agrees with real count. - What do you do after a WBC estimate?
- switch to oil immersion for the 100-cell diff. but FIRST DO A STAIN AND SMEAR EVAL
-
what do you look for when evaluating
-stain
-smear -
Stain: excessively blue, excessively pink, or precipitated stain.
smear: Water contamination, echinocytes everywhere, thickness/length of the smear. - What cells are not included in the 100 WBC count/id?
-
nRBC
Smudge cells
Giant platelet forms -
what do you do if you find a nRBC?
how? -
correct the WBC:
100 x WBC
--------- = Corrected WBC
100 + nRBC -
what do you do when done with the 100 ID/count?
How? -
platelet estimate.
count and avg # platelets in 6 fields, multiply by 15 - when should you repeat a diff?
-
when abnormal cells are seen
nRBC's are seen
immature cells
WBC < 2000/ul