VTMC 337 Midterm
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- EVOLUTION OF BACTERIA...
-
Selection driven by use of antibiotics
Exchange of Plasmids
`` increased stocking density
`` transport between populations over long distances - EVOLUTION OF HOSTS
-
Selection for Performance
`` changes in immunity
`` changes in susceptability -
KOCH'S POSTULATES
SHAKY BECAUSE -
Some species hard to isolate or culture
Some animals silent carriers - INFECTIOUS DISEASES..
-
Complex interatiions between
`` host
`` pathogen
`` environment - PATHOGENICITY...
-
Rare in Bacteria
< 3% are primary pathogens
Most successful bacteria are NOT pathogens - PATTERNS OF SOME BACTERIA AND FUNGAL INFECTIONS...
-
Are changeing over time
Hard to explain
Related to Host Changes
`` immunity
`` genetics
Related to Microbe Changes
`` anitbiotic use - tf selection
`` loss of virulence
Related to Environment Changes
`` husbandry practices -
PATHOGEN
WHAT IS IT
TYPES -
An organism which can cause Disease
Obligate
Primary
Opportunist
Nonpahogen - OBLIGATE PATHOGEN
-
Infectious: 3 - 10
Host predisposition: -
Virulence: ++++
Disease: Distinct
Habitat Diseased or carrier - PRIMARY PATHOGEN
-
Infectious: 100 - 10 to 5
Host predisposition: +
Virulence: +++
Disease: Distinct
Habitat: carrier - OPPORTUNIST PATHOGEN
-
Infectious: > 10 to 5
Host predisposition: ++
Virulence: +
Disease: Nonspecific
Habitat: Normal Flora - NONPATHGEN PATHOGEN
-
Infectious: > 10 to 6
Host predisposition: ++++
Virulence: + / -
Disease: Nonspecific
Habitat: Normal Flora - CONTAGIOUS
-
Spread of pathogen from HOST to HOST
`` directly
`` indirectly - INFECTION
-
Presenxe of a potentially pathogenic organism
Infection does not necessarily imply disease -
ALL INFECTIOUS DISEASES ARE CONTAGIOUS
T/F - False
- VIRULENCE
-
Degree of pathogenicity
`` ie severity of disease
Low numbers cause disease
High rate of reproduction
Strain + Host + Susceptability Factors
`` minor changes in host defenses create major change in apparent virulence
~~ ie some enteric pathogens - PROBIOTIC
- Good bacteria that out competes bad bacteria
- PATHOGENESIS
-
Mechanisms by which bacteria / fungi cause disease
`` includes HOST response to infection
Knowing molecular basis
`` diagnostic measures
`` control measures - INFECTIVITY
- Colonizing Ability
- VIRULENCE FACTORS
-
Determinants of bacteria / fungi which cause disease
Bacterial Virulence
`` polygenic ie multifactorial - ATTENUATION
-
Process of diminishing virulence of organism
Common in developing vaccinations
Note - Attenuation = LIVE - PREDISPOSITION
-
Predisposing Factors
`` make individuals / groups particularly susceptable to infectious diseas
Shipping Fever - Mannhemia haemolytica
`` develops only when all environmental factors are fullfilled -
PATHOGENIC BACTERIA
10 KEY SECRET AGENT SKILLS -
Enter body of Host
Attain unique Niche
Avoid, Subvert or Circumvent Host Defences
Evade Acquired Specific Immune Responses
Multiply or Persist
Cause Tissue Damage or Disease
Exit Host
Transmit Infection to New Host - TOXIC SHOCK SYNDROME
-
Response of host defenses to Superantigen
Superantigen produced by bacteria
`` binds T Cell receptors at variable region (nonspecific) to antigen presenting cells
`` tf high number of T cells activated
`` `` ie 10to5 - 10to6 vs 1 in 10to5
`` tf release of Cytokine Storm
Differences in variable region in individuals
`` individual response to superantigen - REPRODUCTIVE RATES
-
E. coli in vitro
`` 20 - 30 min
Clostridium perfinges
`` 6 min
`` gas producer
Pathogens tend to have higher growth rates
However Mycobacterium paratuberculosis
`` 18 hrs
`` tf chronic disease -
BACTERIAL VIRULENCE
9 CONCEPTS -
Associate with Host
Multiply to Significant Numbers
`` via Attachement
`` via Invasion
Evade Host Defenses
Cause Damage
`` via Toxin Production
`` `` Exotoxin
`` `` Endotoxin
`` via Host Response
`` `` to presence of bacteria
`` `` immune mediated damage -
EPITHELIAL CELL INVASION
GENERAL ASPECTS
IN 3 STAGES -
STAGE 1
Access to Mucosa
`` competion with commensals
`` resistance to host defenses
Adherence
STAGE 2
Cell Damage
Colonization
`` cell penetration
`` intracellular proliferation
`` lateral spread
`` `` cell damage
STAGE 3
Entry into blood stream and lymph
`` resistance to host defenses
`` proliferation
Systemic Spread - BACTERIAL DAMAGE TO HOST
-
Exotoxins
`` local
`` specific effects via specific receptors
`` Inflammatory response to structural components
`` `` cytokines
Endotoxins
`` systemic G-ve
`` Type I Anaphalaxis
`` cytokines
Immune mediated damage -
NEUROTOXINS
TETANUS -
Cell Wall Peptidoglycan
Type II Cytotoxic - HEMOLYSINS
-
Lipoteichoic Acid
`` inflamation
Type III - Immune Complex - LEUCOCIDINS
- Type III - Immune Complex
- ENTEROTOXINS
- Type IV - Delayed Hypersensitivity
- SPECIFICITY
-
Driven by receptor presence
`` Species
`` Organ
Some bacteria are
`` specific
`` `` Strep equi, suis, canis
`` `` Strep equi - throat
`` `` Microsporum canis - skin
`` `` E. coli - intestine
`` some are not
Each host has 20 - 30 pathogens which cause host limited disease
The rest of pathogens are broad host range -
NON SPECIFIC HOST DEFENSES
2 LINES IN THE CORPORAL SAND -
First Line Defenses
`` Surfaces
Second Line Defenses
`` Within Host Tissue -
FIRST LINE DEFENSES
14 IN 3 CLASSES -
PHYSICAL BARRIERS
`` Skin
`` Mucus
`` Mucosal Epi shedding
`` Peristalsis
`` Urination
`` Defecation
`` Sneezing
`` Mucocilary Elevator
CHEMICAL FACTORS
`` Stomach pH
`` Unsaturated Fatty Acids - Skin
`` Lysozyme
`` `` Tears
`` `` Saliva
`` `` Nose
MICROBIAL ANTAGONISM
`` cheer on the home team -
SECOND LINE DEFENSES
10 IN 3 CLASSES -
ANTIBACERIAL PEPTIDES
`` complement lytic complexes
`` complement IgG opsonization
`` transferrin
`` `` sequesters iron
TISSUE PHAGOCYTES
INFLAMMATORY RESPONSE
`` vasodilation tf leakage
`` `` fibrin
`` `` immunoglobin
`` `` complement
`` `` phagocytes
`` systemic mobilization of phagocytes -
SPECIFIC IMMUNE RESPONSE
2 TYPES TARGETING WHO -
TYPE 1 CELLULAR
`` Intracellular Bacteria
`` viruses
`` Intracellular protozoa
TYPE II HUMORAL - Antibodies
`` Extracellular Bacteria
`` Helminths -
GENERAL HOST STATES
THE G8 -
Genetic Makeup of Host
`` some hosts have increased resistance
`` `` ie increase number / diversity of AntiBodies
Nutritional State
`` low Se = high mastitis
`` a few pathogens target well fed fast growing animals
`` `` Clostridium perfinges
Sex
`` Brucella abortus
`` `` dormant in lymph nodes
`` `` gestation - erythrolytic
Pollution
`` ie ammonia gas in barns / stalls
`` `` respiratory problems
Mycotoxins
`` some fungi produce immune suppressors
Foreign Bodies
`` chronic source of infection
`` relapses
`` bacteria protected from lymphocytes in microcrevices
`` water filled capsules
`` `` phagocytes hydrophobic
`` biofilm breaks off forming secondary foci of infection
Age
`` neonates immature immune systems
`` `` foals low interferon for 6 months
`` `` `` tf low cell mediated
`` aged
`` `` reduced immune systems
`` `` increased number of recptors
Stress
`` vastly over rated and undefined
`` ok...
`` sudden changes in feed composition -
HOST DEFENSES
EVASION -
Resist NonSpecific Host Defenses
`` Phagocytosis
`` `` capsules 90% H20
`` `` phagocytes hydrophobic
`` `` tf require AB to bind - typically 7 days
`` `` other surface components interfere with binding
`` Complement
`` `` long LPS chains prevent complement cascade from reaching
`` `` LPS detaches after 5 - 6 components
`` `` `` tf before complement cascade complete
Resist Specific Host Defenses
`` Antibody Resistance
`` `` secrete proteases which destroy AB
`` `` Antigenic variation in daughter cells
`` `` `` tf 7 more days to build new antibody
`` `` `` tf chronic disease -
CPNTAGIOUS DISEASE
CONTROL
MOTHERHOOD STATEMENT -
Contagious disease often follows Multifactorial and Complex events
`` tf no simple formula
Control must be based on Understanding of
`` Unique Host-Parasite Interactions -
DISEASE
TRIAD ONE MORE TIME
WITH KEY FACTORS -
Pathogen
`` Virulence
`` Numbers
Host
`` General State
`` Immunity
`` Nutritional Stress
Environment
`` Temperature
`` Humidity
`` Stocking Rate
`` Mixing
`` Associated Pathogens - AUTOGENOUS VACCINES
-
Culture Specific Pathogen strain(s)
from affected farm
`` will contain specific virulence factors
Kill with fomalin
`` vaccintate animals
Effective for a few years before requiring updates
Usually do not do PCR to identify specific virulence factor
`` that job is left up to the immune system
`` `` ie immune system will have strongest response to most antigenic factors -
SOURCES OF INFECTION
3 -
Endogenous Orgranisms
`` opportunistically pathogenic members of the normal flora
Diseased Animals
`` incubating
`` sick
`` recovered carriers
`` primary or obligate pathogens
Exogenous Sources
`` free living organisms
`` ie Bacillus anthracis -
CARRIERS
DEFINITION -
Individuals that discharge virulent organisms
`` via excretions
`` apparently normal
`` hard to detect -
CARRIERS
4 TYPES
4 EXAMPLES -
Active
`` sick animal
`` Johne's Disease - Colibacillosis
Passive
`` recovered or colonized animal
`` Salmonella
`` `` stressed pigs
Transient
`` organism not established
`` temporarily colonized
`` Shigella spp
`` `` dog drinks from toilet bowl
Latent
`` present in body
`` shed only when immunocompromised
`` Mycobacterium bovis - ROUTES OF INFECTION
-
Direct
`` Animal to Animal
`` Horizontal
`` `` ingestion
`` `` inhalation
`` `` genital
`` `` skin
`` Vertical
`` `` dam to offspring
`` `` `` via egg, fetus, vagina
`` `` sire to offspring
`` `` `` via semen
Indirect
`` air
`` water
`` bedding
`` Vectors
`` `` insects
`` fomites
`` `` Weams boots in the poultry centre -
INFECTIOUS DISEASE CONTROL
5 KEY ELEMENTS -
Detection and Eradication of Carriers
Isolation and Treatment of Sick Animals
Environmental Eradication or Control
Decrease Frequency of Contacts
Increase Resistence
`` Non Specific
`` `` Increase -
INCREASE RESISTANCE
4 WAYS -
Non Specific
`` Increase non specific resistance
`` `` immune modulators
`` `` micro nutrients
`` `` `` Vit A, Se etc
`` Control INtercurrent Disease
`` `` eliminate primary virus or bacteria
`` `` tf avoid secondary infection
`` `` ie clostridum perfungens = diarrhea
`` `` `` + parvo virus = death
Specific
`` Vaccination
`` Genetic selection for resistance -
STAPHYLOCOCCI
VETERINARY SPECIES 4 -
Staphylococcus aureus
Staphylococcus intermedius
Staphylococcus hyicus
Staphylococcus epidermidis -
STAPHYLOCOCCI
HABITAT -
Ubiquitous
`` commensals
`` saprophytes
`` `` (live on inanimate mediums such as my brain)
`` skin
`` mucosal surfaces
Cat and Dog spp similar to human
`` tf cross infection -
STAPHYLOCOCCI
PATHOGENICITY -
Pyogenic
`` pus forming
Require predisposing factors to produce disease
`` must overcome host defenses
`` trauma to skin or mucosa
`` can then travel in blood and seed other sites -
STAPHYLOCOCCI
COAGULASE -
Useful criterion of potential pathogenicity
Positive tend to be pathogenic
`` Dogs - Coagulase+ 95% Stap intermedius
`` Bovine - Coagulase+ 90% Staph aureus
`` Equine - Coagulase+ 90% Staph aureus
`` Feline - Coagulase+ 90% Staph intermedius
Note many Staph spp are Coagulase- -
STAPHYLOCOCCI
RESISTANCE -
Long survival in environment
`` resiste desication for weeks
`` 4 months in dry pus
`` `` tf transmission via flys to damaged skin
`` survive 12% NaCl & 1% phenol for 15 mins -
STAPHYLOCOCCI
MAJOR PATHGENIC FACTORS 3 -
Cellular
Toxins
Enzymes -
STAPHYLOCOCCI
CELLULAR PATHOGENIC FACTORS
6 -
Peptidoglycan
`` inhibist leukocyte migration
`` dermonecrotic
`` activates complement
Teichoic Acids
`` adherence to mucosal surfaces
`` activates complement
Fibronectin Binding Sites
`` enhances adherence to tissues
`` `` exposed dermis
`` Protein A
`` antiphagocytic
`` `` binds to Fx frogment of opsonization site of antibody
`` `` `` tf cant bind phagocyte
`` fixes complement
Capsule
`` antiphagocytic
Staphyloferrin B
`` siderophore
`` `` ie steals Fe from transferrin -
STAPHYLOCOCCI
TOXIN PATHOGENIC FACTORS
6 -
Alpha Toxin (phage mediated)
`` complete hemolysis of sheep and rabit rbcs
`` cytotoxic
`` leucocytotic
`` lyser of platelets
`` disrupter of lysosomes
`` muscle spasms
``histamine release
`` dermonecrosis
`` death
Beta Toxin
`` Incomplete hemolysis
`` leukocyte damage
`` necrosis
Leucocidin
`` leukocyte degranulation
`` `` via membrane permability
Enterotoxins
`` A - Q ie 17 of em
`` nausea
`` vomiting
`` diarrhea
`` SUPERANTIGEN
Toxic Shock Syndrome Toxin
`` enterotoxin F
`` ptoent pyrogenic material
`` SUPERANTIGEN
`` over represented in mastitis strains
Exfoliatin
`` aka Stphylococcal Scalded Skin Syndrome (SSS)
`` damages stratum spinosum junctions
`` `` intrepidermal seperation
`` `` cleft between stratum corneum and underlying epidermis -
STAPHYLOCOCCI
ENZYME PATHOGENIC FACTORS
8 -
Coagulase
`` coagulates plasma
Hyaluronidase
`` diffusing factor - mucinase
Nuclease
`` DNase - destroys DNA
Penicillinase
`` destroys penicillin
Protease
`` destroys proteins
Lipase
`` destroys lipids
`` tf skin damage
Urease
`` splits urea
Spaphylokinase
`` activator of fibrinolysis -
STAPHYLOCOCCI
PATHOGENIC FACTORS
PERSPECTIVE -
Not all factors produced by all strains
Quantitative variation in production amounst strains
virulence cannot be explained in terms of a single factor
Differetn virulaenc mechanisms come int play according to rout of invasion
Express enzymes required for a particular environment -
STAPHYLOCOCCI
HOST DEFENSES -
Phagocytosis
`` main
`` tf if phagocyte deficits highly susceptible
Antibody
`` important
Cell Mediated
`` ?? -
STAPHYLOCOCCI
CATTLE -
Mastitis most economically important
Most common single cause
Acute Inflammatory change
`` peracute with rapid onset
`` usually near parturition
`` large toxin production
`` `` alpha toxin
`` `` fibrinolysin
`` `` coagulase
`` `` lukocidin
`` massive necrosis of tissue
`` `` tf taxaemia
`` early severe febrile response
`` `` abated if gangrenous
Sublclincal Inflamatory Change
`` most common
Chronic Inflammatory Change
`` increase in milk cell counts
`` `` via neutrophil migration
`` Botryomycosis
`` `` chronic pyogranulomatous lesions
`` `` ABs cannot penetrate -
STAPHYLOCOCCI
SHEEP AND GOATS -
Mastitis ala Cattle
Ulcerative Dermatitis -
STAPHYLOCOCCI
SWINE -
Dermatitis aka Staphlylococcal acne
`` Pigs uner 8 weeks
`` small circumscribed pustules over much of boddy
`` also bites on mammaries
Mastitis
`` acute
`` chronic
`` `` more common
`` `` often botryomycotic -
STAPHYLOCOCCI
POULTRY -
S. aureus
Subcutaneous absecesses of foot
`` Bumblefoot
`` synovitis
`` arthritis
`` osteomyelitis
`` `` rare -
STAPHYLOCOCCI
HORSES -
Botryomycosis
`` most common manifestation
`` lesions in spermatic cord
`` castration or penetrating wounds
Dermatitis
`` also common
`` skin wounds
`` photosensitivity -
STAPHYLOCOCCI
DOGS -
Otits Externa
`` infection of external ear
`` Inflammation of Subcutaneous Glands and Tissues
Infection of Skin Glands
`` local cellulitis
`` hyperplasia of skin
`` `` furunculosis
Canine Pyoderma
`` juvinile pyoderm
`` folliculitis
`` cell and AB mediated allery involved in pathogenesis
Cystitis
`` sproadically associated with
`` `` Staph aureus
`` Staph intermedius
`` susceptiblity
`` `` skin folds
`` `` german shepards
SSS
`` generalized exfoliative dermatitis
Infection of -
STAPHYLOCOCCI
CATS -
Secondary Skin Infections
SSS
`` generalized exfoliative Dermatitis -
STAPHYLOCOCCI
RABBITS -
Exudative Dermatitis
Pyemia
`` septicimia in which secondary foci of suppuration occur and multiple abscesses are formed -
STAPHYLOCOCCI
EPIDEMIOLOGY -
Widespread in Animal Environment
Transmission from cow to cow via teat cup liners
`` Development of clinical disease frequently depends on exposure of tissues to stress factors
`` `` recent parturition
`` `` improperly functioning milk machines
Humans are Carriers
`` Nose
`` hands
`` clothes
Endogenous Disease
`` pyoderma
`` otis externa
`` urinary tract infections -
STAPHYLOCOCCI
IMMUNITY -
Toxoid or whole cell vaccines
`` some protective immunity
`` killed s. aureus
`` `` IgG1 but poor opsonization receptors
`` live s. aureus
`` `` IgG2 matches virulence factors
`` beware of claims fo bacterins for dairy herds
Animals recoverd froms tphylocoaccal infections
`` DO NOT acquire long lasting resistance
`` Humoral requires high quantiy of appropriate class and subclass AB
`` cell mediated is quesitonable
Endogenous Vaccines may tilt to cell mediated
`` tf produce long lasting immunity - BACTERIN
- Vaccine consisting of killed bacteria
-
STAPHYLOCOCCI
THERAPY -
Conduct Sensitivity Test First
`` except for ultra acute disease
Chronic Disease
`` expect failure
`` `` fibrous encapsulated deep seated lesions
`` `` prolonged treatment 7 - 11 days
Many strains are Penicillin Resistant
Resistance to ohter ABs
`` aminoglycosides
`` tetracyclines
`` macrolides
`` lincosamides
`` streptogranins
`` not as disseminated as human strains
`` increases in resistance reported -
STAPHYLOCOCCI
HUMAN INFECTION -
Dog Bites
`` high incidence of MRSA (methicillin resistant staph aureus)
Deep Skin Lesions
`` via cattle and sheep
In general intra species spread is limited
`` host specificity -
STAPHYLOCOCCI
FOOD POISONING -
Stphylococcal Enterotoxin
`` secondary contamination of food with human staph
`` occasionally animal staph sp -
STAPHYLOCOCCI
STPHYLOCOCCUS HYICUS -
Exudative Epidermitis aka greasy pig disease
`` Affects very young pigs
``Hair Follicles and Sweat Glands
`` `` excessive sebaceous secretion
`` `` exfoliation
`` `` inflammatory exudate
`` `` parakeratosis and edema
``Oedematus Cnanges
`` `` kidney pelvis
`` `` ureters
`` `` NOT bladder
Eye Infections in Avians
`` associated with coliforms
Source is usually carrier -
STAPHYLOCOCCI
STPHYLOCOCCUS EPIDERMIDIS -
Coagulase Negative !!!
Low grade subclinical Mastitis in Cattle
`` frequently found in high cell count milk
`` may enable infected quarter to withstand infection by other pathogens
`` `` E. coli
`` `` Strep agalactiae
Other staphylococci species are sometimes cause of mastitis
`` ie staph xylosus -
STAPHYLOCOCCI
DIAGNOSIS -
Direct Smears helpful
Culture
Biochemical Tests
And of course the ubiquitous potential significance of
`` isolation of other Staphylococcus spescies in high numbers and / or pure culture - STREPTOCOCCUS
-
sperical or ovoid
Gram +
Chains or pairs
` long chains in broth
Aerobic / Facultatively anaerobic
Pathogenic species fement carbs
Catalase Neg -
STREPTOCOCCUS
CULTURE -
Beta Hemolytic strep
`` are more fastidious in growth requirement
`` generally more virulent
`` blood, serum or animal protein in media
small delicate colonies
`` 1 mm dia
`` smooth
`` glistening
`` some produce a mucoid capsule -
STREPTOCOCCUS
HABITAT -
Commensuals or Parasites
`` mucous memb
`` `` URT
`` `` Lower Genital tract
`` `` skin
`` `` alimentary tract (enterococci) -
HAEMOLYSIS
3 -
Alpha
`` greenish discoloration
Beta
`` wide zone of complete lysis of rbcs
`` medium becomes transparent
Gamma
`` no haemolysis -
STREPTOCOCCUS
ANTIGEN STRUCTURE -
Capsule polysaccharide
Cell Wall Protein Antigens
`` M
`` `` can be shed
`` T
`` R
Group Carbohydrate
`` aka Lancefield groups
`` A to V
Peptidoglycan
`` inflammation -
STREPTOCOCCUS
CELLULAR VIRULENCE FACTORS -
Hyaluronic Acid Capsule
`` host recognizes as self
`` antiphagocytic
`` `` T. pyogenes, S. equi
`` once #s are high turns off expression to save energy
`` `` dry small colonies
M Protein
`` > antigenic types
`` `` tf specific locations ie S. equi Equine Respiratory
`` adherence
`` antiphocytic
Peptidoglycan
`` inflammation
`` fever
`` lymphocte proliferation
`` dermonectrotic -
STREPTOCOCCUS
EXTRACELLULAR VIRULENCE FACTORS -
Streptolysin O
`` lethal for mice, rabbits, guinea pigs
`` oxygen labile
`` Leukotoxic
`` `` PMNs and macrophages
`` `` tf increased host susceptability
Streptolysin S
`` wide zone of haemolysis on aerobic plates
`` oxygen stable
`` Leukotoxic
Steptokinase
`` lysis of fibrin
Dexoyribonuclease
`` may liquify viscous inflammatory exudeate
`` `` tf aids dissemination
Hyaluronidase
`` dissemination factor
`` favars invasion of tissues -
STREPTOCOCCUS
SEROLOGICAL GROUPS -
Group A and B
Lancefield via percipitin test
`` based on dominant carb
C susbstance antigens -
STREPTOCOCCUS
S. pyogenes Group A -
Mainly Human Pathogen
`` septic sore throat
`` scarlet fever
Necrotizing Fascilitis
Toxic Shock like syndrome -
STREPTOCOCCUS
BOVINE MASTITIS
S. agalctiae -
Strep. agalactiae (Group B)
`` typical long coccal chain
`` Beta haemolytic
`` `` narrow zone
`` `` some strains non haemolytic
``CAMP phenomenon positive
`` `` arrowhead appearance
`` `` zone of incomplete straph. aureus haemolysis
`` `` completely lysed by steptococcal haemolysin
Pathogenicity
`` chronic bovine mastitis
`` first attack inflammation
`` some fibrosis
`` subsequent attacks replace secretatory tissue with fibrous CT
`` rarely subacute
`` increased cell milk count
Laboratory Diagnosis
`` CAMP positive
Transmission
`` survives poorly outside of udder
`` tranmsission directtly to teat
`` `` hands
`` `` teat cup liners
`` occasionally indiredt
Immunity
`` increasing frequency with age
`` chronic mild mastitis
`` `` fibrotic change
`` `` watery milk
`` `` tf no age immunity
Therapy
`` penicillin and many other antibiotics
`` `` low development of resistance
`` failure of treatment clinical cases
`` lack of AM penetration in lesions
`` `` blockage of duct
`` `` `` shrinkage of peri ductal fibrous tissue
`` `` `` proliferation of ductal epithelium
`` `` `` or both
Human Infection
`` rarely infects humans
`` neonatal
`` `` septicemia
`` `` meningitis
`` most human strains DISSIMILAR to bovine strains -
STREPTOCOCCUS
BOVINE MASTITIS
Strep. dysgalactiae -
Step. dysgalactiae (Group C, G, L)
Non haemolytic or narrow alpha zone
Location
`` udder
`` and other sites, vagina, tonsil
More acute than Strep. agalactiae but rarely systemic
Often in association with
`` Arcanobacterium pyogenes
`` Peptostreptoccus indolicus
`` faculative anaerobes
`` `` tf bad smell
`` "Summer Mastitis"
`` `` also winter
Transmission
`` flies transmit via exudate
Treatment
`` penicillin per Strep. agalactiae
flies -
STREPTOCOCCUS
BOVINE MASTITIS
Strep. uberis -
Steptococcus uberis
alpha or gamma haemolytic
`` CAMP-Esculin media
`` `` Esculin Split
`` `` 10% strains CAMP positive
Location
`` skin of udder
`` feces
`` tonsil
`` rumen
`` soil
`` `` can reproduce in hay and soil
`` `` tf high enviro #s
sporadic cases of mastitis
`` "environmental mastitis"
Transmission
`` occasionally moderately widespread in herd
`` stress factors
`` `` post parturient
Pathogenicity
`` acute mastits
`` `` soon overcome
`` `` udder appears to revert to normal
Treatment
`` penicillin per Strep. agalactiae -
STREPTOCOCCUS
Strep. bovis -
Streptococcus bovis (Group D)
`` present in mouth and GI Cattle
`` exessive quantities in rumen
`` `` lactic acidosis
`` maybe invoved in feed bloat
`` `` polysaccaride capsule increases
`` `` `` viscosity
`` `` `` foaming properties -
STREPTOCOCCUS
Strep. equi equi -
Steptococcus equi equi (Group C)
Colonies
`` Wide zone beta haemolysis
`` larger
`` honey coloured
`` viscous (when capsulated)
Pathogenicity
`` Specifically an Equine pathogen
`` abscesses in lymph nodes (STRANGLES)
`` `` especially head
`` `` lungs and nasal cavity may be involved
`` highly contagious
`` high (up to 100%) morbidity
`` low mortality
`` Sequelae
`` `` pupura hemorrhagica
`` `` `` type II sensitivity
`` `` `` appears 2 - 3 weeks after apparent recovery
Immunity
`` usually good but temporary after natural disease
Therapy
`` normally sensitive to penicillin
`` AM cannot penetrate abscesses
`` `` tf draining of abscesses may be neccessary -
STREPTOCOCCUS
Strep. equi zooepidemicus -
Streptococcus equi zooepidemicus (Group C)
Colonies
`` Wide zone beta haemolysis
`` tiny < 1 mm
Variety of nonspecific infections in horses
`` ie #1 infection
`` `` trachea
`` `` wounds
`` `` eyes
`` #2 infection
`` `` pleura
`` `` post op
`` `` umbilicus
`` `` uterus
`` joint infections
`` `` very difficult to treat in bone
Also cattle, guinea pigs, rabbits
Important in Western Can Pigs
Occasional cases of human infection reported -
STREPTOCOCCUS
Strep. porcinus -
Streptococcus procinus (Groups E, P, U, V)
Pathogenicity
`` Cervical lymphadenitis
`` `` aka jowl abscess
`` `` aka swine strangles
`` pneumania
`` septicaemia
Midwest USofA mainly
`` rare in Canada -
STREPTOCOCCUS
Strep. suis -
Streptoccus suis
35 casule serotypes recognized
`` many possess Group D antigen
`` but homogeneous spp.
`` `` tf not part of general Group D
Colonie
`` alpha haemolytic
Virulence Factors
`` Capsule
`` `` antiphagocytic
`` `` adherence
`` intracellular survival in non activated macrophages
`` pili via aderence?
Resistance
`` survives in feces up
`` `` 100 days at 0 deg C
`` `` 21 days at 9 deg C
`` rapidly inactivated by disinfectants and cleansers
Affected Species
`` mainly pigs
`` `` esp 5 - 24 weeks
`` rarely isolated from clinical specimens
`` `` cats
`` `` horses
`` `` calves
`` `` sheep
`` `` goat
`` some serotypes are important
`` `` high mortality
`` `` high morbidity
`` `` zoonautics
`` `` `` ie deafness in humans
Pathogenesis Pigs
`` intrapartum or Aerosol (flies)
`` nasal cavities / tonsils
`` ``` main source of dissemination
`` mandibular lymph nodes
`` `` stress via
`` `` `` circovirus
`` `` `` PRRS
`` Septiciemia
`` various organs
`` often involves other bacteria
Clinical Signs
`` variable
`` sudden death without signs
`` high temp - 42.5 deg C
`` anorexia
`` deprssion
`` ataxia
`` paralysis
`` paddling
`` opisthotonos
`` tremors
`` convulsions
`` blindness
`` abortion
Lesions - some of
`` suppurative meningitis
`` pneumonia
`` arthritis (type 9)
`` vegetative endocarditis
`` necrotizing carditis
`` polyserositis
`` skin redding
`` enlarged lymph nodes
`` congestion of parenchymatous organs
Diagnosis
`` culture biochemical tests
`` `` necropsy organs
`` `` nasal, genital secretions
`` heavy frowth of alpha haemolytic Strep from internal organs
`` `` Major Alert
Control
`` minimize stress (ha ha)
`` strategically medicate feed
`` `` prior to known periods of heightened risk
`` `` may just delay clinical cases
`` depopluate and restock
Treatment
`` vaccination results uncertain
`` `` autogenous
`` `` `` pool joints, hearts, brains of several animals
`` `` `` take samples away from lesions
`` penicillin
`` debridment of large amount of tissue
Zoonosis
`` mainly pig workers
`` `` 80% of cases
`` low mortality
`` deafness 57% of cases
`` risk of meningitis -
STREPTOCOCCUS
DOGS AND CATS -
Streptococcus canis (Group G)
`` opportunistic pathogen
`` new born puppies
`` `` septicemia
`` adult dogs and cats
`` `` non specific diseases
`` Necrotizing faciitis and toxic shock like syndrome
`` `` local infection and sudden death
`` `` `` usually constant pain
`` `` `` treat via debridement of large amount of tissue
`` `` `` Prior treatment with quinalone
`` `` `` `` enable phage tf superantigen
`` usually just abscesses
Zoonosis
`` dog and cat strains appear different from human -
STREPTOCOCCUS
Enteroccocus spp. -
Canine Urinary tract
GI tract of man and animals
Increasing importance in nosocomial infections
Septicemia in Chickens
Antimicrobial Resistance
`` major problem in humans
`` esp Enterococcus vancomycin resistance (VRE)
Enterococcus durans
`` recently described in diarrheic illness
`` foals
`` calves
Streptococcus pneumonia
`` 80 types
`` 50% mortality in humans
`` primarily human pathogen
`` meningitis in calves
`` mastitis cows
`` occasional pneumonia
`` `` calves
`` `` dogs
`` `` horses
`` Monkeys - tf zoonosis
`` `` pneumococcal pneumonia
`` `` speticemia
`` `` meningitis
`` `` acute course high mortality
`` Horses
`` `` LRT inflammation
`` `` URT commensual in some - CORYNEBACTERIA
-
Gram Positive
Chinese letter Palisade form
Pleomorphism
`` occasionally marked
`` `` ressemble cocci
Non sporing
Animal Pathogins
`` non motile
`` pyogenic
Aerobic or microaerophilic
Veaded Staining
`` metachromatic granules with methylene blue -
CORYNEBACTERIA
Arcanobacterium pyogenes -
Epidemiology
`` survives on
`` `` normal mucous membrane
`` `` skin
`` mainly opportunistic pathogen
Diagnosis
`` gram stain
`` `` G+ rods to cocci
`` `` palisade - Chinese letters
`` culture
Immunity
`` toxoid used with limited results in prevention of infection
`` whole culture antigens (bacterins)
`` `` limited value
Treatment
`` penicillin normally quite effective in vitro
`` `` may be infective in vivo
`` `` `` must penetrate fibrous capsule and pus
`` drainage is extremely important -
CORYNEBACTERIA
Arcanobacterium pyogenes
CATTLE -
Suppurative processes in almost every tissue or organ
`` esp if organ compromised
Ofen found with other bacteria
`` esp anaerobic
`` `` F. necrophorum
`` `` P. indolicus
Lesions often found
`` lungs
`` `` secondary purulent bronchopneumonia follow virus
`` `` `` Mycoplasma
`` `` `` Pasteurella
`` `` `` Mannheimia
`` `` `` parasitic infection
`` Liver
`` `` abscesses
`` `` F. necrophorum more common in cattle
`` Uterus
`` `` abortion
`` `` purulent metritis
`` Joints
`` `` young calves
`` `` `` post umbilical infection
`` `` adults
`` `` `` post metritis or mastitis
`` Heart
`` `` endocarditis
`` `` myocarditis
`` `` traumautic pericarditis
`` Bone Marrow
`` `` young calves
`` `` `` osteomyelitis post umbliliculitis
`` `` adults
`` `` `` vertebae post foot lesions
`` Sinuses
`` `` frontal purulent post dehorning
`` Vesicular semminitis
`` Mastitis
`` `` rare lactating cow
`` `` Dry Cow or Heifer associated with
`` `` `` Strep. dysgalactiae and/or P. indolicus
`` Abortions
`` `` high #s in abomasal fluid -
CORYNEBACTERIA
Arcanobacterium pyogenes
Sheep and Goat -
Lung
`` secondary purulent bronchopneumonia following
`` `` Mycoplasma
`` `` Pasteurella
`` `` Mannheimia
`` `` parasitic infection
Joint and Foot infections
Occasionally causes abortion -
CORYNEBACTERIA
Arcanobacterium pyogenes
SWINE -
Lung
`` secondary purulent bronchopneumonia following
`` `` Mycoplasma
`` `` Pasteurella
`` `` Mannheimia
`` `` parasitic infection
Subcutaneous abscesses
`` associated with skin trauma ie tail biting
`` development of pyaemia and production of secondary lesions
`` `` lungs
`` `` bone marrow
Metritis
`` post partum
`` purulent -
CORYNEBACTERIUM
Renale Group -
Corynebacterium renale
Corynebacterium cystitidis
Corynebacterium pilosum
Specific invaders of unrinary tract of Cattle
`` cystitis and pyelonephritis
`` rarely found in other animals
Serotypes vary in virulence by species - ie specificity
`` C. pilosum
`` `` cystitis but NOT pyelonephritis
`` C. renale
`` `` chronis cystitis AND pyelonephritis
`` C. cystitidis
`` `` chronis cystitis AND pyelonephritis
`` `` clinically most severe
`` `` most difficult to treat with AM agents
C. pilosum and C. renale
`` frequently found as normal flora
`` `` lower urogenital tract healthy cows (rarely isolated)
`` `` prepuce of bulls
C. cystitidis
`` `` normal flora prepuce of bulls
Culture
`` normal media
`` blood agar and/or serum enhances growth
`` G+ rods to cocci palisade
Biochemical
`` all 3 spp catalase +
`` all 3 spp STRONG producers of urease
Resistance
`` probably low outsided of body
Virulence Factors
`` Pili
`` `` adherence
`` Urease
`` `` produces ammonia
`` `` `` inactivates complement
`` `` `` direct tissue damage (pH 9)
Pathogenesis
`` adherence overcomes urinary washout
`` `` enables ASCENDING urinary tract infection
`` `` alkaline inflammation and tissue destruction
`` incidenece of disease much higher in female than male
`` `` higher postpartuent
`` `` `` pressure effect of uterus reduces urine flow
Epidemiology
`` spread via external genitalia
`` `` venereal
`` `` tail to tail contact of purulent material
`` `` `` via tail swishing during urination
Diagnosis
`` urine sediment showing
`` `` typical organisms
`` `` pus cells
`` Culture #s important
`` `` C. renale & C. pilosum
`` `` `` normal flora
`` `` `` `` tf > 100 cfu significant
`` `` C. cystitidis
`` `` `` not normal flora
`` `` `` `` tf < 100 cfu Significant
Treatment
`` sensistive to penicillin
`` chronic cases may need prolonged treatment
`` moderate damage in bladder
`` `` recurrence
`` `` secondary infection
`` `` `` Proteus
`` `` `` E. coli
`` `` `` Streptococci -
CORYNEBACTERIUM
pseudotuberculosis -
aka Corynebacterium ovis
`` caseous lymphadenitis sheep and goats
`` `` Thin Ewe Syndrome once visceral
`` ulcerative lymphadinitis and deep abscesse of horses
`` rarely lulcerativ and/or pyogranulomatous lesions bovine and alpaca
Culture
`` G+ rod to cocci
`` `` palisade - Chinese Letter formation
`` slightly haemolytic on blood agar
`` colonies dry appearance
Virulence Factors
`` Surface Liped
`` `` pervents or delays phagosome-lysosome fusion
`` `` `` tf intraphagocyte survival (unactivated)
`` `` `` Facultative Intracellular Parasite
`` Exotoxin
`` `` Phospholopase D aka Hemolysin
`` `` `` hemolytic via increase in vascular permeability
`` `` serine protease
Restistance
`` survives up to 2 months in environment
`` `` esp moisture and organic material
Pathogenicity
`` Contagious Caseous Lymphadenitis Sheep
`` Primary lesion
`` `` most frequently in lymph nodes
`` `` `` esp prescapular or prefemoral
`` `` `` also liver, lungs and associated lymph nodes
`` infection enters via
`` `` damaged skin
`` `` `` shearing, docking, castration, dipping
`` `` respiratory Tract
`` `` pyaemia may develop later
`` Early Lesions
`` `` soft greenish yellow pus
`` `` followed by enlargement of lesion or lymphnode
`` `` `` inspissated pus
`` `` `` laid in concentric circles - onion
`` `` oleder lesions are grey
`` animals may remain in good condition despite lesions
`` `` eventually become emaciated
`` `` `` Thin Ewe Syndrome
Diagnosis
`` Typical Caseous Lesion
`` `` almost pathonomic
`` `` note similar lesion via Actinobacillus ligieresii
`` `` `` differentiate via gram stain
`` `` `` `` G- morse code
`` sspirate lymph nodes
`` `` organisms hard to find
`` culture
`` ELISA
Immunity
`` Cell mediated - major role
`` also antitoxin antibodies
Vaccine
`` commercial vaccine
`` `` sheep and goats
`` `` reported good protection
`` `` special adjuvants
`` autogenous vaccines do not work well
Treatment
`` AM effective in vitro
`` less effective in vivo
`` `` penetration of absceses
`` `` introcellular location
`` low resistance
`` hygiene
`` isolation
`` avoid rough food
`` drain abscesses
`` cull positives
`` vaccinate
`` disinfect wounds -
CORYNEBACTERIUM
psuedotuburculosis
HORSES -
Ulcerative Lymphangitis in legs
`` `` pus from lymphatic vessels on legs
`` `` similar to reportable GLANDERS
`` `` `` Burkholderia mallei
`` `` `` G-
Contagious Acne
`` aka canadian Horse Pox
`` `` uncommon folliculitis
Chest abscesses
`` pigeon chest via specific lymph node -
CORYNEBACTERIUM
psuedotuburculosis
CATTLE -
Skin infections
`` sporadic
Mastitis
`` sporadic -
CORYNEBACTERIUM
psuedotuburculosis
HUMAN -
Lymphadenitis
`` post animal contact
`` few cases reported - RHODOCOCCUS equi
-
Pyogenic organism
`` mainly affects foals
`` sproadic cases in many other spp.
`` `` including human
Culture
`` Weakly G+ short rod to cocci
`` sometimes partly acid fast
`` non haemolytic
`` mucoid colonies
`` `` white at 24 hrs
`` `` redish pink 14 - 72 hrs
`` specific "shower" smell
Resistance
`` highly resistant to dessication
Habitat
`` soil organism
`` feces of herbivores
`` multiplies in well manured soils
`` multipiles in foal intestine
`` `` not completely anaerobic environment
`` `` faculative aerobes
`` `` `` survives but do not reproduce in anaerobic environment
`` world wide distribution
Virulence Factors
`` strain variation
`` Vap surface protein (A and B)
`` intra macrophage survival via
`` `` corynemycolic acids, phospholipids, glycolipids
`` `` induce granuloma formation
`` polysaccharide capsule
`` `` inhibits pulmonary macrophage oxidative burst
`` Exoenzymes aka equi factors
`` `` phospholipase C
`` `` cholesterol oxidase
Pathogenicity
`` Faculative Intracellular Parasite
`` suppurative chronic bronchopneumonia
`` `` mainly young foals (1 - 6 months)
`` purulent infiltration of lymph nodes
`` intestinal ulceration
`` `` abscess fromation mesenteric lymph nodes
`` swine
`` `` submaxillary lymph nodes
`` `` `` produce small abscesses
`` `` sybmaxillar lymph nodes
Epidemiology
`` serious problem on endemic farms
`` `` poor hygiene, old farms
`` Morbidity up to 90%
`` Mortality 5%
Immunity
`` ceell mediated and humoral
`` only ACTIVATED macrophages kill efficiently
`` ingestion and killing via PMN and macrophages
`` `` significantly increased by presence of specific antibodies
Diagnosis
`` Radiograpy
`` Direct Smear
`` Culture
`` ELISA
`` PCR
Treatment
`` Erythromycin - rifampin Combination
`` `` lipophilic prolonged treatment
`` Clarithromycin and rifampin (?)
`` Azithomycin
`` Ceftiofur
`` Tilmicosin
`` `` high [lung]
`` `` `` tf works despite high MIC
Prevention
`` Hygiene
`` `` reduce environmental load is key
`` EHV-2 predisposing factor
`` Endemic Situations
`` `` hyperimmune plasma
`` `` prophylatic AM
`` ensure colostrum intake
`` dust control
`` remove foals from contaminated grounds -
LISTERIA monocytogenes
Cattle and Sheep
3 Manifestations -
Encephalitis
Septicaemiia
Abortion -
Listeria monocytogenes
Cattle and Sheep
Septicemia -
Young Ruminants or Monogastrics of all ages
Enters through alimentary canal / rumen
Death is rapid
Multifocal Hepatitis may be seen -
Listeria monocytogenes
Cattle and Sheep
Abortion -
Organism localized in uterus
`` via bacteremia
`` placentitis
`` `` fetal septicemia
`` `` `` abortion
`` `` `` still birth
`` `` `` sick neonate
Sheep, cattle and other species
Cattle
`` abortion 4 - 8 months
Sheep
`` abortion terminal stages
Normally cow or ewe little effected -
Listeria monocytogenes
Cattle and Sheep
Mastitis - Very rarely described in cattle
-
Listeria monocytogenes
Swine -
Infection rarely reported in pigs
Occasional cases of septicemia
`` usually in young animals
Very rarely nervous form
Stillbirths and late abortions have also been recorded -
Listeria monocytogenes
Poultry -
Secondary to Primary Agent
`` parasitic
`` virus
Carcass
`` ofen debilitated
`` edematous
`` may be focal hepatitis
`` may be necrotic myocarditis -
Listeria monocytogenes
Other Animals -
Described in more tha 50 species
`` wild
`` birds
`` humans -
ERYSIPELOTHRIX
2 SPECIES -
Erysipelothrix rhusiopathiae
Erysiopelothrix tonsillarum -
ERYSIPELOTHRIX rhusiopathie
Gen -
Type species of genus
> 24 serotypes recognized
Primarily associated with infection of swine
`` Swine Erysipelas
Also disease in
`` Lambs
`` adult Sheep
`` Turkeys
Occasionally disease in
`` cattle
`` dogs
`` mink
`` horses
`` avian spp
`` mice
`` human
Present in surrounding slime of various fish -
ERYSIPELOTHRIX rhusiopathie
Morph and Cult -
G+ slender rod
`` non motile
`` encapulated
`` `` more capsule = more virulence
`` non sporing
`` Aerobic
`` `` faculative anaerobe
`` rough and smooth forms
Culture
`` Smooth colony
`` `` alpha (greenish) hemolytic
`` `` no growth MacConkey
`` `` 0.8 mm dia
`` `` small straight rods
`` `` organisms from ACUTE cases
`` `` more virulent
`` Rough colony
`` `` alpha (greenish) hemolytic
`` `` no growth MacConkey
`` `` Pleomorphic and filamentous
`` `` `` up to 60 microm
`` `` organisms from
`` `` `` chronic lesions
`` `` `` repeated passage invitro of smooth
`` `` less virulent than smooth -
ERYSIPELOTHRIX rhusiopathiae
Resistance -
Resists
`` drying
`` pickling
`` salting
`` smoking
Long survival in putrefying carcases
Survive in feces
`` up to 6 months -
ERYSIPELOTHRIX rhusiopathiae
Virulence -
Capsule
`` antiphagocytic
Neuraminidase
`` production of enzyme may correlate with virulence -
ERYSIPELOTHRIX rhusiopathiae
Pathogenicity - 4 -
Primarily Swine
Acute Septicemia
`` 3 - 12 months
`` `` decline Maternal AB
Subacute Skin Lesions
`` urticarial aka DIAMOND
`` AG/AB complexes in subQ vasculature
`` almost pathonemonic
`` similar to Swine Fever Virus
`` `` also maintains appetite
`` `` tf differentiate via penicillin
Chronic
`` Endocarditis
`` arthritis
Abortion
Note
`` course of disease depends markedly on
`` `` virulence of strain
`` `` resistance of host -
ERYSIPELOTHRIX rhusiopathiae
Acute Disease -
Clinical
`` Fever
`` Anorexia
`` depression
`` vomition
`` stiff gait
Frequently fatal if untreated
Red Patches on skin
`` esp ears, abdomen, medial thigh
Spleen and Lymph nNodes
`` enlarged
`` reddened
Mucosa of Stomach and Intestine
`` inflammed
`` sometimes hemmorrhagic
`` may be ulcerated -
ERYSIPELOTHRIX rhusiopathiae
Skin Form -
Less Acute
`` may recover without treatment
DIAMOND SHAPED LESIONS
`` red or purple
`` may be elevated
`` several cm
`` back and abdomen
`` May become necrotic -
ERYSIPELOTHRIX rhusiopathaie
Chronic From -
Endocarditis
`` typical vegetative lessions on heart valves
`` `` exp mitral (left AV)
Mainly observed in Swine
Also
`` sheep
`` cattle
Excercise Intolerance
`` sudden death when moved -
ERYSIPELOTHRIX rhusiopathaie
Arthritic -
Chronic
`` degenerative changes in joints
Often seen in mature animals
`` can occur in young
Severe Lameness
`` even though mild lesions
Proliferation of synovium
`` conjestion
`` increase in synovial fluid
`` `` slight turbidity
`` `` NOT purulent
Ulceration of joint surface cartilage
`` inflammation at base of ulcer
Severe Cases
`` ankylosis
Lesions may result from
`` Antigen Persistence in situ
`` `` induces immune mediated damage -
ERYSIPELOTHRIX rhusiopathaie
Sheep -
Normally Polyarthritis
`` 2 - 3 month lambs
`` lesions per swine
Older sheep
`` cause of post dipping lameness
`` `` acute cellulitis of lower limbs
`` `` mulitplies in organic matter in dip water
`` `` penetrates small wounds in skin -
ERYSIPELOTHRIX rhusiopathaie
Cattle -
Arthritis
`` rarely -
ERYSIPELOTHRIX rhusiopathie
Poultry -
Turkeys more commonly affected
`` mature birds
`` more males
`` `` via fighting
Acute Septicemia
Cyanosis
`` blue comb
Large hemorrahges
`` heart muscles
`` legs
`` serous membranes
Similar acute disease occasionally seen in
`` ducks
`` chickens
`` pheasants
`` other birds - ERYSIPELOTHRIX tonsillarum
-
Dogs
Arthritis
Endocarditis -
ERYSIPELOTHRIX rhusiopathiae
Human -
Usually self limiting cellulitis
`` erysipeloid
`` following skin wound
Rare Cases of
`` septicemia
`` endocarditis
`` arthritis -
ERYSIPELOTHRIX rhusiopathaie
Fish -
Non Clinical Infection
Found in surface slime
`` human risk for
`` `` erysipeloid lesions -
ERYSIPELOTHRIX rhusiopathaie
Marine Mammals - Septicemia
-
ERYSIPELOTHRIX rhusiopathaie
Epidemiology -
Organism passed in feces and urine of healthy pigs
Present in health pig
`` tonsils
`` gallblader
Carrier animals may subsequently
`` show clinical disease
Survives many months
`` carcasses of affected animals
Survives outside of body
`` via flies, dried blood
`` weeks summer
`` months winter
Infection of susceptible swine
`` mainly oral
`` possibly transcutaneous
Turkeys
`` contaminated semen
`` fight wounds -
ERYSIPELOTHRIX rhusiopathaie
Predisposing Factors -
Environmental Stress
Dietary Change
Fatigue
Sublclinical aflatoxicosis -
ERYSIPELOTHRIX rhusiopathaie
Diagnosis -
Direct Grams Stain and Culture
`` skin biopsy from both sides of lesion
`` spleen
`` liver
`` lung
`` arthritis
`` `` several joint samples
Can be hard to culture
`` tf ELISA for ABs -
ERYSIPELOTHRIX rhusiopathaie
Immunity -
Developed Immunity is Good
`` rare for second occurance of acute disease in recovered animal
Hyperimmune serum
`` produced from horses
`` 2 week period of protection
Attenuated vaccine strains available
Killed Vaccines (aka Bacterins) available
Antibody opsonins important
Phagocytic function
`` via Mononuclear phagocytes
`` NOT via PMN (neutrophils) -
ERYSIPELOTHRIX rhusiopathaie
Control -
Quarrantine Replacement Animals
`` minimum 30 days
Proper disposal of infected carcasses
Vaccination recommended
`` areas with pervious history
`` multiple serotypes
`` tf use 2 vaccines to cross protect -
ERYSIPELOTHRIX rhusiopathaie
Treatment -
Penicillin
`` highly effective
Tylosin
Tetracyclines - GENUS BACILLUS
-
Aerobic (facultative) spore bearing bacilli
`` G+ or G variable
Long threads
`` forms rhizoid colonies
Usually Motile
Catalase Positive
Most are saprophytes
`` capable of living in innanimate media
Bacillus Anthracis
`` highly pathogenic
Many organisms of group
`` air
`` soil
`` water
`` dust
`` wool
`` feces
`` dried grass and straw -
BACILLUS Anthracis
Morph and Cult -
Rod shaped
`` 3 x 0.4 to 9 x 2 microm
`` parallel sides
`` ends usually
`` `` straight
`` `` slightly curved
In vitro
`` single and diplobacillary
In vivo
`` long chains (boxcar)
Agar Colony
`` ground glass surface
`` waxy appearance at edge
`` aka Medusa Head appearance
Forms Capsule
`` esp tissue
`` blood agar
`` > 20% CO2 -
BACILLUS Anthracis
Resistance -
Sporulated Forms
`` considerable resistance
`` 10 mins boiling water survives
`` 30 min boiling water kills
Spores only form
`` presence of oxygen
`` specific nutrients
`` DO NOT form in UNOPENED carcasses
Spores
`` generally very resistant to disinfectants
`` `` except oxidizing Agents
`` `` `` KMnO4, H2O2
`` viable in soil
`` `` > 60 yrs -
BACILLUS Anthracis
Virulence -
Poly D glutamate Capsule
`` antiphagocytic
`` antiopsonizing
`` plasma mediated
Tripartite Toxin
`` Edema Factor (EF)
`` Protective Antigen (PA)
`` Lethal Factor (LF)
`` plasma mediated
`` `` different plasmid
EF + PA = Edema
PA + LF = Lethal
PA + LF + EF = Edema and Lethal
EF and LF REQUIRE PA for activity
Toxin major systemic damage in host
`` vascualar permeability
`` capillary thrombosis
`` due to induction of generalized release
`` `` IL-1 -
BACILLUS Anthracis
Pathogenesis -
Most common routh of infection
`` Oral
`` organism present in
`` `` soil
`` `` `` contaminated pasture
`` `` food stuffs
`` `` `` contaminated MBM
Some cases via
`` infected wounds
`` arthropod bites
Bacillus enters
`` phrynx (tonsil)
`` small intestine
`` often temporarily held up in local lymph nodes
`` `` toxins produced stimulate edema
`` `` `` death via asphyxiation if lymph node in throat
Alternatively
`` septicemia
`` death via systemic toxin production
Incubation period
`` 1 - 5 days
Course of Disease
`` few hours to 2 days
`` throat form
`` `` may live several days
`` `` mainly seen in pigs
`` septicemic form
`` `` animal dies within a few hours
`` `` `` 48 hrs max
Toxin damages endothelium
`` severe edema
`` hemorrhage via
`` `` nose
`` `` GIT
`` `` Urinary Tract
`` -
BACILLUS anthracis
CATTLE -
Typically acute septicemic disease
If suspected DO NOT open carcass
`` otherwise release massive #s of organism
`` `` will sporulate on exposure to air
`` otherwise
`` `` cannot sporulate
`` `` overcome by other putrifying bacteria
Gross enlargement of spleen
Petechiae and ecchymoses on serous membranes
General toxic degeneration of parenchymatous organs
Absence of rigor mortis -
BACILLUS anthracis
Pigs -
Acute Form
`` excess fluid in abdomen
`` `` clear or slightly blood tinged
`` severe conjestion of SI and draining vessels
`` necrotic mesenteric lymph nodes and draining area
`` general lesions of toxemia
Throat Form
`` edema of throat
`` `` may extend down nect
`` submaxillary lymph nodes
`` `` necrosis
`` `` possible hemmorhage
`` Death via asphyxiatin
`` `` via obstructive edema -
BACILLUS anthracis
Sheep - Similar to Cattle
-
BACILLUS anthracis
Horses - Throat or Septicemic form
-
BACILLUS anthracis
Dog -
Throat Form
or
Chronic carbuncel like lesion in mouth -
BACILLUS anthracis
Diagnosis -
Cattle
`` aspirate blood from superficial vessel
Pigs
`` swab endematous area in throat
Treat smears HgCl2 1:1000 for 5 min before staining
Polychrome Methylen Blue
`` square ended
`` pinkish purple capsule
Gram stain
`` G+ rods
Clostridia
`` round ended
`` no capusule
`` `` except Clostridum perfringens
`` may form spores -
BACILLUS anthracis
Immunity -
Antibody to toxin protects
Vaccination
`` "avirulent" spore vaccine available
`` do not use AM for 4 - 5 days post vaccination
`` annual booster for populations at risk
`` do not use within 60 days of slaughter for food -
BACILLUS anthracis
Treatment -
Sensitive to Penicillin
Illness to far advanced to treat in most cases
Humans
`` suspicion of GI or Inhalation
`` `` immediate AM treatment -
BACILLUS anthracis
Control -
Reportable Disease
`` local vet
Destroy
`` Carcass
`` in contact material
`` burning or deep burial
Prevent spore formulation
`` do not open carcass
`` disinfect premises
Fumigate or Sterilize
`` clothes
`` instruments
Soil contamination
`` immunize stock
Quarantine
`` suspected animals
`` in contact animals - BACILLUS cereus
-
Cattle
`` acute gangrenous mastitis
`` `` Uncommon
`` `` sometimes fatal
`` implicated in sporadic abortion
Sheep
`` implicated in sporadic abortion
Human food poisoning
`` some strains
`` enterotoxin
`` usually not severe - BACILLUS licheniformis
-
Abortion in cows
`` increasing isolated
`` source may be heavy growth in silage -
ENTEROBACTERIACEAE
Family -
Large group of G- bacilli
`` essentially intestinal parasites
`` `` also includes sprophytics
Some are highly pathogenic
Many are opportunist invaders
Non Sporing
Non motile
`` peritrichate flagellae
Easily grown on media
Aerobic
`` faculatative anaerobes
All species
`` ferment glucose
`` `` with acid production
`` `` commonly gas
`` Oxidase Negative
`` some ferment lactose
`` `` Escherichia
`` `` Klebsiella
`` `` Enerobacter
`` some are Non Lactose Fermenters
`` `` Salmonella
`` `` Proteus
`` `` Yersinia
`` `` Shigella
`` `` `` variable by spp.
Genera in Family
`` 28
Many of these organisms are normal GI flora
Initial Identification
`` colony morphology
`` Colour on MacConkey
`` `` lactose fermentors RED - ESCHERICHIA coli
-
Normal Flora
`` 10>5 / g in feces
to Extreme pathogen
Relatively resistant to drying
`` 3 months
Survives and multiplies in environment
Hundreds of serotypes -
ESCHERICHIA coli
Cultural -
G- bacilli
Marked wide zone beta (complete) hemolysis
`` blood agar
`` some strains
`` hemolysis tends to be associated with
`` `` pathogenicity -
ESCHERICHIA coli
Resistance -
Resists Heating
`` 60 deg C 15 mins
`` some strains
Drying
`` moderate resistance
Killed
`` disinfectants
`` pasteurization -
ESCHERICHIA coli
Ecology -
Intestinal Organism
Survives out side host
`` > 6 months
`` `` in manure
`` `` `` detection indicates fecal contamination -
ESCHERICHIA coli
Antigenic Structure -
O Antigens
`` cell wall
`` LPS
`` aprox 180
H antigens
`` pili
`` `` flagellin protein
`` > 50
`` Pigs
`` `` F4 (K88 old)
`` Cattle
`` `` f5 (K99 old)
K antigens
`` Capsule
`` `` polysaccharide or protein
`` > 100 -
ESCHERICHIA coli
General Virulence Factors -
Capsule
`` Antiphagocytic
`` `` important in septicemic strains
`` capsule functions as adhesins
`` `` only a few strains
Endotoxin
`` LPS important virulence factor
`` `` septicemia
`` `` mastitis
`` `` endometritis
`` strong host reaction
`` `` Fever
`` `` hypotension
`` `` `` shock
`` `` complement activiation
`` `` endothelial damage
`` `` abortion (secondary)
`` `` lekopenia
`` `` `` followed by leuocytosis
siderophores
`` septicemic strains
`` `` often carry Col V plasmid
`` `` `` genses for
`` `` `` `` serum resistance
`` `` `` `` iron uptake -
ESCHERICHIA coli
Pathogenicity -
Responsible for many enteric infections in young animals
`` esp
`` `` calves
`` `` young swine
`` `` lambs
`` `` puppies
`` also acute speticemia in young
Sequelae
`` meningitis
`` polyarthris
`` endocarditis
Important cause of mastitis in cattle
`` mild chronic
`` severe acute fatal
`` `` usually within 2 weeks of calving
`` sheep and sows also affected -
ESCHERICHIA coli
Neonatal Disease -
Septicemic
`` no ingestion of colostrum
`` `` tf no transfer of maternal immune globulins
`` infection through
`` `` umblilicus
`` `` GIT
`` rapid invasion
`` death within 24 - 48 hrs
`` septicemic lesions
`` `` liver, lung, heart, kidney, spleen, meninges
`` `` `` can culture large #s immediately post mortum
`` More common in calves than piglets
Diarrhic
`` most common form
`` `` both calves and piglets
`` diarrhea prominant feature
`` most common < 1 weak
`` also older animals esp piglets to immediate post weaning -
ESCHERICHIA coli
Diarrheic Disease Pathotypes -
aka pathovars
Enerotocigneic - ETEC
Shigatoxin producing - STEC
`` includes Enteropathogenic - EPEC
Enterohaemorrhagic - EHEC
ENEROINVASIVE - EIEC
Enteroaggregative - EAggEC
NECROTOXIC - NTEC -
ESCHERICHIA coli
ETEC -
Enterotoxigenic
Usually species specific E.coli serotypes possessing
`` Adhesive Pili
`` `` plasmid mediated
`` `` attachement to jejunum and ileum
`` Enterotoxins
`` `` ST
`` `` LT
STa
`` Heat stable toxin
`` activates quanylcyclase in intestinal mucosa
`` inhibits influx of Na and Cl ions
`` stimulates serction of Cl and bicarb ions
`` tf decreased absorption and increased eflux of water
STb
`` stimulates increase in PGE2 levels
`` `` induces deodenal and jejunal secretion
`` `` `` water
`` `` `` electrolytes
LT
`` heat labile toxin
`` stimulates adenylcyclase
`` `` decreases absorption of sodium
`` `` increases efflux of
`` `` `` Na
`` `` `` Cl
`` `` `` water -
ESCHERICHIA coli
Enterotoxigenic Serovars
Adhesins and Hosts
Sadly have to "know this" -
Adhesin / Host
F4 (K88) / Pig
F5 (K99) / Calf, pig, lamb
F6 (987P) / Pig, calf
F18 / pig
F41 / calf, pig
AIDA 1 / pig
Some strains produce more than one fimbrial antigen
`` could be an antigen that vaccines or PCRs dont cover
`` `` tf Autogenous vaccine
Enterotoxigenic presentation has also been reproted in dogs and horses
`` rare -
ESCHERICHIA coli
Enteropathogenic -
EPEC
These strains attach intimately to
`` surfaces of intestinal epi cells
`` `` eface microvilli
`` `` cause cells to form actin pedestals
Affected Spp
`` RABBITS
`` dogs
`` pigs
`` humans
`` other animals
Lesion
`` attaching and effacing
`` collapse of microvilli
Intestinal Location
`` varies with species
Virulence Factors
`` Adhesion to intestinal epi cells
`` `` via bundle forming pilus
`` `` `` plasmid coded bdf gene
`` `` pili attach to particular intestinal cells
`` closer attachment via intimin protein
`` `` chromosomal gene eae
`` `` `` E. coli attaching effacing -
ESCHERICHIA coli
Shiga Toxin Producing -
STEC
`` includes Enterohemorrhagic (EHEC)
Produce attached and effacing lesions
Also produce hemorrhagic diarrhea
Most recognized serotype
`` 0157:H7
`` `` hamburger disease
`` `` hemolytic uremic syndrome in humans
`` `` high number of receptors for toxins in kidneys
`` `` 10 bacteria infective in susceptible humans
`` `` `` host genetic factors
Note other strains produce verotoxins
`` hemorrhagic diarrhea
`` `` cattle
`` `` pigs
`` `` cats
`` `` dogs
`` `` rabbits
`` `` some zoonotic
`` `` `` can be carried by healthy animals
Virulence Factors
`` Shiga Like toxins
`` `` STx1
`` `` STx2
`` `` Varients
`` `` `` STx2c
`` `` `` STx2d
`` `` `` STx2e
`` `` `` `` 0139 Edema disease pigs
`` `` `` STxf
`` `` baceriophage coded
`` Action of these toxins
`` `` local via endothelial cells
`` `` systemic via endothelial cells in other sites mainly
`` `` `` kidney
`` `` `` brain
`` `` -
ESCHERICHIA coli
Edema Disease -
Unusual GIT infection in SWINE
`` not classified as enteropathogenic
Caused mainly by serovoars
`` 0138
`` 0139
`` 0141
These Strains produce verotoxin
`` STx2e
Absorbed toxin damages endothelium
`` produces edema
`` `` intestine
`` `` stomach
`` `` mesentery
`` `` Sub Q
`` `` Brain
Characterized by NERVOUS signs
Associated with post weaning
Some Edema Disease E. coli strains may also be
`` Entertoxigenic
`` `` tf function as both
`` `` `` ETEC E.coli and Edema Disease E.coli -
ESCHERICHIA coli
Enteroinvasive -
EIEC
Destroys enterocytes
`` Dysentery syndrome humans
Rare in animals -
ESCHERICHIA coli
Enteroaggregative -
EAggEC
Diarrhea in
`` children
`` piglets
`` rabbits?
`` other animals? -
ESCHERICHIA coli
Necrotoxigenic -
NTEC
Produce cytotoxic necrotizing factors
`` CNF1
`` CNF2
Diarrhea
`` pigs
`` calves
`` dogs
`` rabbits?
`` horses? -
ESCHERICHIA coli
Dogs and Cats -
Urinary Tract Infections (UTI)
`` Specific strains
`` relapse common
`` `` Invasion of bladder epi
`` `` `` tf reemerge after high Vd AM treatment
Pyometra
`` associated with pseudopregnancy in bitches
Granulomatous colitis
`` boxer dogs
`` specific strains
Non specific
`` wound
`` navel
`` skin
Neo natal diarrhea
Post porturient metritis -
ESCHERICHIA coli
Poultry -
Infection of egg yolk
`` bacteria penetrates shell
`` embroy dies or
`` hatches sick
`` `` death up to 3 weeks after hatching
Infection of serous membranes in broilers
`` often secondary to other agents
`` `` ie Mycoplasma
`` `` ie Infectious Bronchitis Virus
`` pale yellow white fibrinous material
`` `` surface of air sacs and pericardium
`` `` joint cavities
Septicemia
`` high mortality
Cellulitis
`` rapid growth genetics
`` `` weakened skin immunity allows entry
Coligrnuloma -
ESCHERICHIA coli
Foals -
Septicemia
`` inadequate colostrum
`` `` systemic spread
`` diarrhea? -
ESCHERICHIA coli
Epidemiology -
Varies with species and disease
Essentially enteric organism
`` but can survive outside host for several months
`` easily contaminated enviromnent
`` easy oral ingestion
Pathogenic strains
`` find a suitable environment in animal
`` multiplication
`` become dominant species -
ESCHERICHIA coli
Diagnosis -
Direct Smear
`` SMALL intestine
`` `` jejunum #s normally LOW
`` `` `` > 100/oilfield Significant
Culture - Biochemical tests
`` serotype best if possible
Demonstration of enterotoxins
`` immunologic methods
PCR Detection of Genes encoding
`` enterotoxins
`` shiga-like toxins
`` other virulence factors
`` via culture from feces
Isolation from Organs
`` must be from from fresh dead in order to be significant -
ESCHERICHIA coli
Control -
Hygiene
Management
`` avoid crowding
Vaccines for most common serotypes
`` F4
`` F5
`` F6
`` F41
`` used in pregnant animals
`` `` tf colostral protection neonates
`` oral vaccination of piglets
`` killed pathogenic strains
`` `` in feed
`` `` prevent post weaning diarrhea
`` `` extreme situations -
ESCHERICHIA coli
Treatment -
Particulrly caplbe of developing multiple AM resistance
`` tf sensitivity testing
`` transferable
`` `` tf Narrowest spectrum possible
Approach Each outbreak via sensitivity testing
`` sensitivity results may not match therapeutic results - SALMONELLA
-
Ubiquitous Parasite
`` human
`` animals
`` birds
`` reptiles
Major Zoonotic
`` agent of human food poisoning
Grouping
`` O Antigen
Specific identity
`` flagellar antigens
Two Species
`` Salmonella enterica
`` Salmonella bongori
Most Clinical Salmonellae strains
`` S. enterica subspp I
> 2300 Serovars (serotypes)
Motile via peritrichate flagellae
`` except
`` `` S. enterica S. Pullorum
`` `` S. enterica S. Gallinarum -
SALMONELLA
Culture Morph -
G- Bacilli
MacConkey
`` non lactose fermenters
`` pale colonies
`` `` not definitive -
SALMONELLA
Ecology -
Intestinal Organism
`` not regarded as normal intestinal flora -
SALMONELLA
Antigenic Structure -
Very Complex
Primary Grouping
`` O antigens
`` `` somatic antigens
`` `` `` arabic numerals
Secondary Subgrouping
`` Serovars (species)
`` based on H antigens
`` `` may be diphasic
`` `` `` ie flagellae may assume two forms
`` `` `` `` Form 1 Specific
`` `` `` `` `` small letters
`` `` `` `` Form 2 Group
`` `` `` `` `` arabic numerals
Vi Antigen (somatic)
`` additional to O and H
`` intraperitneal virulence mouse
`` `` Vi antiserum passively protects mice against living virulent typhoid bacilli
`` `` O antiserum only protects against endotoxin
`` Vi Antigen associated with protection against
`` `` phagocytosis
`` `` bactericidal effect of serum
Kauffman White Scheme
Groups
`` A, B, C1, C2, D
Sero Types ie
`` S. Typhimurum and S. Newport
`` ``non host adapted
`` S. Gsllinarum and S. pullorum
`` `` reportable disease
`` `` non motile
`` `` `` key differentiator
O Antigens (Cell Wall)
H Antigen
`` phases 1 and 2 -
SALMONELLA
Dieases 3 -
Septicemia
`` localized lesions
`` `` abortion
`` `` `` cattle sheep horses
`` `` embolic pneumonia
`` `` `` mainly pigs
Acute Enteritis
`` ileitis-colitis
Chronic Diarrhea
`` wasting
Disease Type Depends on
`` dose
`` strain virulence
`` host susceptability
`` `` age
`` `` immune status
Recovery from clinical disease
`` asymptomatic carrage state
`` `` months to years
`` `` tf INTERMITTENT shedding
`` `` `` esp stressed -
SALMONELLA
Pathogenesis -
Initial Event
`` colonization of SI
`` `` attachment mediated by
`` `` `` at least 3 different adhesins (fimbriae)
Second Event
`` mucosal invasion
`` `` ileum and colon
`` `` via brush border
`` `` severe inflammation lamina propria
`` `` `` host adapted types
`` `` `` `` peyers patches
`` `` `` `` mesenteric lymph nodes
`` `` `` `` liver
`` `` `` `` septicemia
`` `` survival in phagocytic cells
`` `` `` some strains are facultative intracellular parasites
Infection most commonly oral
`` consumption of contaminated food -
SAMONELLA
Epidemiology -
Capable of surviving and multiplying in environment
Large number of recovered or latent carriers
`` turkeys - 70%
`` Broilers - 50%
`` Hogs - 20%
`` Dairy Cow - 12%
`` Beef Cow - 3%
`` Horses - 20 - 70%
`` `` esp anesthesia stress
`` Dogs - 1 - 36%
`` Cats - 0 - 14%
tf extensive sources of infection
Excretion
`` feces
`` milk
`` `` especially at partuition
`` eggs
`` feeding habits create high exposure -
SALMONELLA
Diagnosis -
Culture - Biochemical tests
Final serotyping
Dection of antibodies against
`` O and H antigens
Negative
`` requires Three consecutive cultures
ELISA
`` not accurate for S. suis spp.
PCR
`` feces enrichment broth -
SALMONELLA
Immunity -
Humoral and cell mediated important
Killed Vaccines
`` proven low efficacy
Attenuated Virulent Strains
`` most efficient -
SALMONELLA
Treatment -
Antibiotic Sensitivity Testing
`` highly reccomended
Effective AMs
`` Fluoroquinolones
`` Ampicillin
`` Trimethroprim
`` Tetrachylines
`` `` usually effective
AM use controversial
`` use only when systemic clinical signs ? -
SALMONELLA
Control -
#s are everything
`` tf Management
`` tf Hygiene
Vaccination
`` killed vaccines useless
`` attenuated live can be effective
`` `` S. suis vaccine reported to work
AMs
`` high resistance
Intestinal Flora Seeding
`` probiotics
`` works for chickens
`` mixed results horses
Reportable Diseases
`` Fowl Typhoid
`` `` S. Gallinarum
`` Pullorum Diseases
`` `` S. Pullorum
Reptiles
`` S. arizona spp.
`` `` poultry - SALMONELLA enterica subsp arizonae
-
aka S. Arizona
Often produces acid in lactose
`` but not dulcitol
`` ferments malonate
`` `` key differentiator
Specific diarrheal disease in turkeys
`` also
`` `` nervous signs
`` `` blindness
`` transmission
`` `` farm to farm
`` `` via eggs
Main Hosts
`` reptiles
`` carriers or clinical disease
`` `` depends on numbers
Pathogenicity
`` Normally fatal in turkey poults
`` `` 3 - 21 days
`` `` may also affect 3 - 4 weeks
`` Humans rarely
`` `` Septicemia
`` `` pyemia
`` contaminated food -
SHIGELLA
Whole enchilada -
G- bacilli
Species
`` Shigella dysenteriae
`` Shigella flexnerii
`` Shigella boydii
`` Shigella sonnei
Resistance is not high
Virulence factors
`` cytotoxin (shiga-toxin)
`` `` plasmid mediated cell wall antigens
`` `` enable invasion and survival inside phagocytes
Pathogenicity
`` important in primates
`` rarely reported other animals
`` `` diarrhea
`` isolated from feces of clinically normal dogs
`` `` transient excreters
`` very low #s for infection
`` invades enterocytes
`` `` tf dysentry
Epidemiology
`` as per other enterobacteriaceae
Diagnosis
`` specific bacteriology
Treatment
`` AM sensitivity testing
`` Resistance
`` `` sulphonamides
`` `` ampicillin
`` `` tetracycline
`` `` `` some strains
`` often transferable -
KLEBSIELLA Pneumoniae
Cult Morph -
G-
`` usually highly capsulated
`` non motile
Culture
`` non hemolytic
`` mucoid to watery via capsule
`` `` non mucoid variants occur
`` MacConkey
`` `` mucoid lactose fermenter -
KLEBSIELLA pneumoniae
Antigenic Structure -
Well defined capsular antigens
`` 77 in all
O antigens
`` share many properties with
`` `` E.coli O antigens -
KLEBSIELLA pneumoniae
Virulence -
Capsule
`` prevents phagocytosis
`` attachment
`` esp Mare GI
Pili
Exotoxin -
KLEBSIELLA pneumoniae
Pathogenicity -
Considerable variation between types
Does NOT depend only upon presence or absence of big capsule
Bovine
`` mastitis
`` `` indistinguishable from E.coli mastitis
`` usually aquired via woodsavings or sawdust
Equine
`` metritis
`` abortion
`` `` serotypes mainly
`` `` `` 1, 2, 5
`` neonatal septicemia
`` `` arthritis
`` `` `` must sensitivity test
`` pnemonia
`` `` rarely
`` veneral transmission
Swine
`` acute post parturient mastitis
`` `` similar to E.coli type
Dogs
`` urogenital tract disease
`` otitsi externa
`` pneumonia
`` `` rarely -
KLEBSIELLA pneumoniae
Epidemiology -
Survives
`` feces
`` soil
Most Commonly saprophyte of Genital Tract
`` may be transferred venerally in horses
`` stallion or mare carriers -
KLEBSIELLA pneumoniae
Diag Immun Treatement -
Diagnosis
`` Culture
`` may not be certain of association with disease
Immunity
`` little knowledge of specific immunity
Treatment
`` AM sensitivity test
`` usually sensitive to
`` `` Gentamicin
`` `` Chloramphenicol -
ENTEROBACTER
W enchilata -
G-
``bacilli
`` motile
`` often capsulated
7 Species
`` `` lactose fermenters
Rarely pathogenic
`` associated with diverse infections in dogs and cats
`` `` cholecystitis
`` `` pyothorax
`` `` UTI
`` `` bacteremia
`` Cows
`` `` occasionally mastitis -
PROTEUS
W. enchilata -
G- rod
`` Non lactose fermentor
`` charcteristic swarming on agar
`` `` swimming via flagella
Pathogenicity
`` typically opportunistic
`` Strong urease producer
`` `` tf UTI
`` `` urolithiasis
`` pneumonia
`` otitis externa
`` `` esp dogs
Habitat
`` primarily GI
`` decomposing animal organic matter
Treatment
`` AM sensitivity REQUIRED
`` multi resistant -
YERSINIA
Resistance -
Feces Survival
`` long at low temp -
YERSINIA pseudotuberculosis
Pathogenicity -
Facultive Intracellular Parasite
`` as are all vet spp.
`` killed by neutrophils
`` NOT killed by macrophages
Produces diseas in wide variety of animals
`` mainly
`` `` guinea pigs and other rodents
`` also affects
`` `` cats
`` `` zoo carnivores
`` `` birds
`` `` swine
`` `` muskox
`` sporadic outbreaks
`` `` chinchilla and mink
`` Rarely affects
`` `` sheep, cattle, horses
`` `` `` Genital system
`` `` `` `` abortion
`` `` `` `` neonatal death
`` `` `` `` epididymo-orchitis
`` human
`` `` mesenteric lymphadenitis children
`` `` septicemia adults
Pathogenies
`` Depends on numbers
`` Oral
`` `` no lesions
`` `` `` carrier
`` `` `` `` stress
`` `` `` `` `` enteritis
`` oral
`` `` enteritis
`` `` `` regional lymph nodes
`` `` `` `` tubercle like lesions
`` `` `` `` liver, spleen other organs
`` `` `` `` `` tubercle like lesions
`` `` acute septicemia
`` `` `` death -
YERSINIA pseudotuberculosis
Epi Diag Immun Treatment -
Epidemiology
`` widely distributed in nature
`` `` alimentary tract of normal animal
`` `` `` pathogenic under stress conditions
`` zoonatic
Diagnosis
`` culture and biochemical tests
`` from lesions
Immunity
`` Vaccination rarely used
`` low virulence live organisms
`` `` interparenteral inoculations
Treatment
`` Chlortetracycline
`` streptomycin
`` chloramphenicol -
YERSINIA entercolitica
Epidemilogy -
GI tract animals
Sources of Drinking water
Most often isolated pathogenic serotypes
`` carried in Normal GI pigs
`` `` also cattle, dogs, cats
Serological Evidence
`` little relationship between animal and human strains -
YERSINIA enterocolitca
Antigenic Structure -
> 30 Serotypes
Most common isolates in clincal cases
`` 0:3
`` 0:5
`` 0:8
`` 0:9
`` );27 -
YERSINIA enterocolitca
Pathogenicity -
Infectious Dos
`` 10>8 to 10>9
`` refigerated food
`` `` seafood
`` `` icecream
Causes
`` lymphadenitis
`` ileitis
`` `` ocassionally ulcerative enteritis
`` diarrhea
`` fever
`` abdominal pain
`` `` humans
`` `` chinchilla
`` `` young dogs
`` `` cats
`` `` goats
`` `` pigs
Systemic Disease
`` less capable than Y. pseudotuburculosis
`` remarkably associated with serotype 0:8 -
YERSINIA enterocolitica
Diagnostic -
Special CIN culture medium
`` enhances isolation
Feces
Lymph node biopsy
Parenchymal organs -
YERSINIA enterocolitica
Treatment -
Fluroquinolones
Trimethoprim-sulfonamides
Tetracycline - YERSINIA pestis
-
G-
`` safety pin morphology
`` `` significant but not pathonomonic
Causes Bubonic Plague
`` swollen lymph node
`` rat flea borne
Endemic Sylvatic infection W USofA ground squirrels
`` may spread via fleas to dogs and cats and to humans
`` `` cases of infection via handling infected tissue
`` cats highly susceptable
`` `` highly virulent to humans via droplets
`` `` `` tf pneumonic presentation
`` `` `` `` systemic within 2 days
`` Black Death
`` `` via endotoxin mediated gangrene
Septicemia, lymphadenitis, pneumonia
2 isolations in Canada
`` bushy tailed BC woodrats
Beware Recently arrived from SW USofA Cats
`` `` swollen lymph nodes in head
`` `` `` several day incubation period
Some resistant strains emerging -
CAMPYLOBACTER
GENUS -
G- Slender Rods
`` 0.2 to 0.5 mm long
`` spiral appearance in longer forms
`` comma or SEAGULL shape in shorter forms
Domestic Animals
`` GI
`` Genital -
CAMPYLOBACTER fetus subsp venerealis
Morph Cult -
G- Slender Rods
`` 0.2 to 0.5 mm long
`` spiral appearance in longer forms
`` comma or SEAGULL shape in shorter forms
Culture
`` Microaerophilic
`` slow growth (2-5 days)
`` Translucent dew drop colonies
`` `` up to 1 mm dia
`` `` may be rough
`` `` `` multiple morphology in a given culture
`` 25 - 37 deg C -
CAMPYLOBACTER fetus subsp venerealis
Virulence -
S Layer surface protein
`` antiphagocytic
`` antigenic variation
`` `` tf chronic infection
`` `` `` esp bull prepuce
Cell Wall LPS
No exotoxins -
CAMPYLOBACTER fetus subsp venerealis
Pathogenicity -
Cows
`` Infertility
`` `` early embryonic death and reabsorption
`` `` subsequent chronic metritis (months)
`` `` `` increased very late returns
`` Abortion
`` `` small %
`` `` `` usually 4 - 6 months
Bulls
`` carried on preputial mucosa
`` survives for years
`` no lesions of significance
First Evidence of Disease
`` increased #s cows returning to estrus late in breeding season -
CAMPYLOBACTER fetus subsp venerealis
Epidemiology -
Venereal Spread
Bulls
`` persistent asymptomatic carriers
Beef Herds
`` cow infertilty may lead to use of 2 or 3 x normal bull number
Cow can carry organism for short period
`` tf infect new bulls -
CAMPYLOBACTER fetus subsp venerealis
Diagnosis -
Cow
`` microscopic examination of
`` `` cervico-vaginal mucus
`` vaginal mucus agglutination test
`` `` more value for herd test
Smears
`` fetal stomach contents
`` cotyledons
`` stain
`` `` Methylene Blue
`` `` Gram
`` `` FAT
Culture
`` aborted fetal stomach contents
`` `` usually pure culture
`` cotyledon
`` `` may be contaminats
`` cervical vaginal mucus
`` `` may be contaminated
`` hard to grow
`` `` requires transport medium
PCR
`` emerging
Bull
`` sheath washings
`` F.A. Test
`` Culture
`` `` enrichment transport medium required -
CAMPYLOBACTER fetus subsp venerealis
Immunity -
Vaccines
`` formalin killed (aka bacterin)
`` suspended in oily adjuvatn
`` useful
`` `` probably need to revaccinate annually
`` result in IgG opsonins
`` `` imobilize and clear bacteria from genital tract
Cure Claim for vaccination of bulls -
CAMPYLOBACTER fetus subsp venerealis
Treatment -
Streptomycin parenterally effective in cows
`` but rest for a few months
`` `` also clears infection in most animals
Bull
`` sheath washing with streptomycin is effective
`` semen often treated with
`` `` penicillin and streptomycin - CAMPYLOBACTER fetus fetus
-
G- Slender Rods
`` 0.2 to 0.5 mm long
`` spiral appearance in longer forms
`` comma or SEAGULL shape in shorter forms
Culture
`` Microaerophilic
`` slow growth (2-5 days)
`` Translucent dew drop colonies
`` `` up to 1 mm dia
`` `` may be rough
`` `` `` multiple morphology in a given culture
`` 25 - 37 deg C
Serologically and Biochemically distinct from
`` C. fetus venerealis -
CAMPYLOBACTER fetus fetus
Pathogenisis -
Cause of Abortion
`` sporadic in Cattle
`` outbreaks in sheep
`` usually last third of pregnancy
Growth stimulated by substance in
`` fetal cotyledons
`` ovine liver
`` ovine kidney
Sometimes delivery of weak lambs at term
Feces
`` fomites
`` `` Ingestion
`` `` `` Bacteremia
`` `` `` `` Gestational Uterus
`` `` `` `` `` Metritis / Placentitis
`` `` `` `` `` `` Late Abortion -
CAMPYLOBACTER fetus fetus
Epidmiology -
Environmental Transmission via Ingestion
`` NOT venereal
Survives
`` gall bladder of some sheep
`` feces of sheep
`` `` esp young animals
`` magpies transfer flock to flock
`` contaminated shepard boots -
CAMPYLOBACTER fetus fetus
Diagnosis -
Isolation of organism from fetus
`` stomach contents
`` lungs
`` placenta
Culture
`` aborted fetal stomach contents
`` `` usually pure culture
`` cotyledon
`` `` may be contaminats
`` cervical vaginal mucus
`` `` may be contaminated
`` hard to grow
`` `` requires transport medium
PCR
`` emerging
Doughnut Shaped Necrotic Foci
`` sometimes found on liver of fetus
`` indicative of infection in sheep -
CAMPYLOBACTER fetus fetus
Immunity -
Vaccination with formalin killed (bacterin)
`` protects against synchronous challenge
`` partially protects animals infected 1 month PREVIOUSLY
Abortion completely or almost completely prevented -
CAMPYLOBACTER fetus fetus
Treatment and Control -
Streptomycin Effective
`` may reduce number of abortions
`` `` must be used whole flock in face of outbreak
Oral treatment chlortetracycline effective
Isolate aborting ewes
Move pregnant ewes to clean areas -
CAMPYLOBACTER fetus fetus
Zoonosis -
May infect humans
Usually
`` infants
`` pregnant
`` elderly
Abortion
Brucellosis like disease
Septicemia -
CAMPYLOBACTER jejuni
Morph and Cult -
G- Slender Rods
`` 0.2 to 0.5 mm long
`` spiral appearance in longer forms
`` comma or SEAGULL shape in shorter forms
Culture
`` Microaerophilic
`` slow growth (2-5 days)
`` Translucent round colonies
`` `` often mucoid
`` `` up to 1 mm dia
`` `` `` multiple morphology in a given culture
`` 37 - 42 deg C
`` no growth at 25 deg C -
CAMPYLOBACTER jejuni
Pathogenisis -
Leading cause of diarrhea in humans
`` chickens and turkeys
`` `` 80 - 100% carriers
Diarrhea in
`` dogs
`` cats
`` calves
`` sheep
`` mink
`` lab animals
`` more severe in young animals
Hemorrhagic diarrhea often present in
`` dogs
`` cats
`` mink
Cattle
`` sproradic abortion outbreaks
`` mastitis
Sheep
`` abortion
Chicken
`` "vibrionic hepatitis" old reports
`` `` up to 35% decrease egg production
`` `` hemmorrhagic necrotic liver
Isolation from
`` normal pigs
`` `` intestinal contents and feces
`` normal cattle
`` `` gall bladder
`` `` milk
`` `` feces
`` normal dogs
`` `` feces
`` normal sheep
`` `` feces
`` normal monkeys
`` `` feces
`` pigeons and many other birds
`` `` feces
Note closely related C. upsaliensis
`` associated with diarrhea in dogs
`` isolated from asymptomatic dogs and cats
`` `` tf zoonotic risk -
CAMPYLOBACTER jejuni
Epidemiology -
World Wide Distribution
Oral infection
`` contaminated feed
`` water
`` hands in humans
Zoonotic Disease -
CAMPYLOBACTER jejuni
Diagnosis -
Culture from animals showing fever and diarrhea
`` feces
`` intestinal contents
`` special selective media
`` `` otherwise filter through 0.65 microm filter
`` `` `` blood agar
Direct smear
`` feces
`` 5 or more SEAGULLS per oil field
`` `` suggestive -
CAMPYLOBACTER jejuni
Treatment -
Erthromycin
Tetracycline
Chloramphenicol
`` useful in dogs -
HELICOBACTER
Habitat -
Different Species
`` different host ranges
`` `` species specificity to 1 - 3 hosts
`` different primary sites
`` `` mainly GI -
HELICOBACTER
Pathogenisis -
Chronic Gastritis, gastroduodenal ulceration, adenocarcinoma, eteritis, hepatitis
`` humans
`` `` not all strains pathogenic
`` controversial dogs and cats
`` `` many causes
`` `` `` dietary, allergy, food intolerance etc
Clinical Signs
`` vomiting
`` melena
`` `` acute episodes gastric bleeding
`` weight loss -
HELICOBACTER
Diagnosis -
Clinical Signs
`` Vomiting
`` melena
`` Weight loss
Biopsy results
`` patchy distribution
`` `` tf use endoscope
Culture
`` very difficult
PCR
`` genus only
Common in healthy dogs and cats
`` could be variety of Helicobacter like organisms
`` varying pathogenicity
Dogs and cats with gastric enteritis
`` some respond to treatment for H. pylori
`` some do not
Urease Test
`` H. pylori produces high amount of urea
`` `` survival in stomach -
HELICOBACTER
Zoonosis - 5 -
H. pylori
H. bizzozeronii
H. heilmannii
H. Canis
H. pullorum
Note low potential for zoonosis
`` human infections likely from environment -
HELICOBACTER
Treatment -
Eliminate all other causes of gastritis
Verify presence of Helicobacter
AM Combinations
`` amoxicillin or tetracycline
`` `` plus metronidazole
`` bismuth subslicylate and ranitidine
`` proton pump inhibitors
`` `` omeprazole
Long treatments
`` tf 2 AMs
`` `` avoid selection of resistant mutants -
LAWSONIA intracellularis
Morph and Cult -
Porcine Proliferative Enteropathy Complex
G-
`` curved or rod shaped
Culture
`` Microaerophilic
`` cell culture only -
LAWSONIA intracellularis
Pathogenisis -
Porcine Proliferative Enteropathy Complex
Includes
`` Porcine Intestinal Adenomatosis (PIA)
`` Necrotic Enteritis (NE)
`` Proliferative Hemmorrhagic Enteropathy (PHE)
`` thickening of mucosa of SI and LI
`` `` Enterocyte Proliferation
Clinically
`` PIA
`` `` anorexia and failure to sustain growth
`` `` No diarrhea
`` NE
`` `` persistent scours
`` `` `` no blood but mucus present
`` `` progressive wasting
`` PHE
`` `` sudden death
`` `` projectile bloody diarrhea
`` `` anemia
`` `` cases melena
Triggered by stress -
LAWSONIA intracellularis
Epidemiology -
World Wide Distribution
Non hemmorhagic presentation
`` often undetected clinically
`` lesions detected at slaughter
`` `` up to 28%
PIA and NE
`` mainly 6 - 20 weeks
`` common in purchased feeder pigs
`` clinical signs
`` `` 3 - 14 days after movement
PHE
`` mainly young adults
`` repopulated units and breeding stock
Other animal species may bring into high health herds -
LAWSONIA intracellularis
Diagnosis -
No effective method for PE in individual live pigs
Necropsy observation of lesions
Histo
`` intracellular organisms
`` `` acid fast stain
`` silver stain
PCR
Serology
Immunoflurescent AB
Immunohistochemistry