Biochemistry Video 02
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- What is ATP?
- Adenosine nucleoside with three phosphate groups attached to the 5' carbon on the sugar
- What are nucleotides attached together by?
- phosphodiester bond
- What is a nucleic acid?
- long chain of nucleotides
- In what direction is a phosphodiester bond made?
- It takes the 3' OH from the existing sugar and binds it to the 5' phosphate group of the incoming sugar.
- How do we read DNA or RNA?
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5' to 3'
At the beginning of DNA there is a loose phosphae group because there is no previous nucleotide's OH to bind to. - By convention, in which direction is nucleotide order?
- 5' to 3'
- What base always binds with Thymine?
- Adenine
- Which base always binds with cystine?
- guanine
- What are the bonds between AT and GC nucleotides known as and how many bonds are there between the respective groups?
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There are two hydrogen bonds between A and T.
There are three hydrogen bonds between C and G - What type of DNA is most of our DNA?
- B-DNA
- What is the significance of the major and minor grooves?
- they act as receptors for DNA Binding Proteins, which regulate the transcription of DNA
- How do we measure the denaturation of DNA when it is being heated?
- Measure the absorbance of light. As it dissosiates it will absorb more light because the base pairs are showing and absorbing the light.
- how is the melting point of a strand of DNA determined.
- It is the temperature at which half of the DNA is denatured.